Tag
MFa1-Secre

Part:BBa_K792002

Designed by: Manuel Giménez   Group: iGEM12_Buenos_Aires   (2012-09-24)

Secretion tag from yeast α-factor mating pheromone (MFα1)

This part is the secretion signal peptide from the yeast α-mating factor. This signal peptide directs the secretion of the produced protein, and therefore allows the exportation of it. This peptides are cleaved once the protein is in the lumen of the ER.


To use this tag, attached it directly to your proteins CDS.


See also: A Kozak sequence from α-factor mating pheromone (MFα1) BBa_K792001
This parts was originally built to be used in iGEM BsAs 2012 project to implement a cross-feeding regulatory system between two different yeast strains with specific auxotrophy. Visit our wiki to read details about the designing process and implementation details of our system. Also feel free to contact us for any question regarding this part. Igem.bsas.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/yeast
//proteindomain/localization
Parameters
None