RBS
MFa1-Kozak

Part:BBa_K792001

Designed by: Manuel Gimnez   Group: iGEM12_Buenos_Aires   (2012-09-24)

Kozak sequence from yeast α-factor mating pheromone (MFα1)

The Kozak sequence is the eukaryotic analog of the bacterial RBS, it is short sequence that includes the ATG, required for proficient initiation of translation. It is known that the 5’UTR sequence of the MFα1 gene of yeast produces efficient initiation of translation.

This part is that specific region of the MFα1 gene.

See also: A yeast secretion tag from α-factor mating pheromone (MFα1) BBa_K792002
This parts was originally built to be used in iGEM BsAs 2012 project to implement a cross-feeding regulatory system between two different yeast strains with specific auxotrophy. Visit our wiki to read details about the designing process and implementation details of our system. Also feel free to contact us for any question regarding this part. Igem.bsas.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/yeast
Parameters
None