Coding
yA

Part:BBa_K678035

Designed by: DTU-Denmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-04)

yA (green pigment) from Aspergillus nidulans

The gene yA from Aspergillus nidulans encodes a p-diphenol oxidase (laccase) that converts a yellow pigment precursor to the mature green form (1).


Usage and Biology

The A. nidulans conidia (asexual spores) contain a dark green pigment in their walls. The pigment protects the spores from damage by ultraviolet irradiation. The synthesis of this pigment involves the expression of several genes (ωA, yA andfiA) and other genes can modify the amount- and chemical properties of the pigment (dilA, chaA, drkA). A yA mutant produces yellow spores (1).


Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1176
    Illegal XbaI site found at 380
    Illegal PstI site found at 1540
    Illegal PstI site found at 1927
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1176
    Illegal NheI site found at 1742
    Illegal PstI site found at 1540
    Illegal PstI site found at 1927
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1176
    Illegal BamHI site found at 1453
    Illegal BamHI site found at 1736
    Illegal XhoI site found at 87
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1176
    Illegal XbaI site found at 380
    Illegal PstI site found at 1540
    Illegal PstI site found at 1927
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1176
    Illegal XbaI site found at 380
    Illegal PstI site found at 1540
    Illegal PstI site found at 1927
    Illegal NgoMIV site found at 1577
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 54
    Illegal BsaI site found at 1616
    Illegal EcoRI site found at 1176
    Illegal XbaI site found at 380
    Illegal PstI site found at 1540
    Illegal PstI site found at 1927


[edit]
Categories
//chassis/eukaryote
//direction/forward
//function/reporter/pigment
Parameters
chassisAspergillus nidulans