Protein_Domain
SbpA

Part:BBa_K525401

Designed by: Timo Wolf   Group: iGEM11_Bielefeld-Germany   (2011-09-10)

S-Layer SbpA from Lysinibacillus sphaericus

Bielefeld-Germany2011-S-Layer-Geometrien.jpg

S-layer SbpA from Lysinbacillus sphaericus

S-layers (crystalline bacterial surface layer) are crystal-like layers consisting of multiple protein monomers and can be found in various (archae-)bacteria. They constitute the outermost part of the cell wall. Especially their ability for self-assembly into distinct geometries is of scientific interest. At phase boundaries, in solutions and on a variety of surfaces they form different lattice structures. The geometry and arrangement is determined by the C-terminal self assembly-domain, which is specific for each S-layer protein. The most common lattice geometries are oblique, square and hexagonal. By modifying the characteristics of the S-layer through combination with functional groups and protein domains as well as their defined position and orientation to eachother (determined by the S-layer geometry) it is possible to realize various practical applications (Sleytr et al., 2007).


Usage and Biology

S-layer proteins can be used as scaffold for nanobiotechnological applications and devices by e.g. fusing the S-layer's self-assembly domain to other functional protein domains. It is possible to coat surfaces and liposomes with S-layers. A big advantage of S-layers: after expressing in E. coli and purification, the nanobiotechnological system is cell-free. This enhances the biological security of a device.

This S-layer gene is in the Freiburg BioBrick Assembly standard so functional protein domains can easily be fused to its C- or N-terminus. SbpA was characterized with BBa_K525405.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 32
    Illegal BglII site found at 149
    Illegal XhoI site found at 1924
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 3121
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 421
    Illegal BsaI.rc site found at 550


[edit]
Categories
//chassis/prokaryote/ecoli
//proteindomain/internal
Parameters
n/a S-Layer SbpA from Lysinibacillus sphaericus