Project
SbpA|LigA

Part:BBa_K525006

Designed by: Manuel Wittchen   Group: iGEM11_Bielefeld-Germany   (2011-10-25)


Fusion Protein of S-Layer SbpA and LigA

Fusion Protein of S-Layer SbpA and LigA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 104
    Illegal BglII site found at 221
    Illegal BglII site found at 4611
    Illegal XhoI site found at 1996
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 76
    Illegal AgeI site found at 5209
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 493
    Illegal BsaI.rc site found at 622
    Illegal SapI.rc site found at 4825


[edit]
Categories
//cds/enzyme/ligase
//chassis/prokaryote/ecoli
//proteindomain/internal
Parameters
biologyS-Layer
chassisE. coli
controlPT7
originLysinibacillus sphaericus, Escherichia coli