Generator

Part:BBa_K494002

Designed by: Haissi Cui, Tilman Flock, Sebastian Gude, Christoph Hartlmüller, Florian Praetorius, Jan Schüürmann, Tobi Wauer, Philipp Wortmann   Group: iGEM10_TU_Munich   (2010-10-25)

Arabinose-inducible mCherry Generator
In general we want to provide a new, scalable principle of gene regulation based on termination and antitermination which can be further developed, tested and optimizted by everybody. To enable that in the easiest way possible we decided to not just add the switches we designed but instead the material needed for evaluation. Therefore we focus on providing the parts needed for verification and testing of new individual switches.

With this BioBrick we provide a backbone which allows further cloning to test individual switches which can be easily designed using the principles described in the TU Munich iGEM 2010 wiki.

BBaK494002 is intended as a positiv control for use with BBa_K494001.

This is a ready to use composite part consisting of the backbone BBa_K494001, a modified version of pSB1A10, the PBad Promotor BBa_I13453 and the reporter protein mCherry BBa_J06702 including RBS and double Terminator BBa_B0015. BBa_K494001 serves as a positive control: Without a terminator in between eGFP and mCherry and an additional promoter in front of mCherry, BBa_K494002 allows measuring mCherry fluorescence upon induction and comparison to the eGFP fluorescence. With BBa_K494001, measurements can be standartized to an internal positive control and it allows verification of induction, measurement consitions and expression kinetics.

Since BBa_K494001 can be used for evaluation of terminators and PoPS devices in general, BBa_K494002 can be used as a control in all those applications.

Emission spectra of induced and uninduced pSB1A10mod positive control BBa_K494002 ; A: eGFP fluorescence ex: 501 nm, B: mCherry fluorescence ex: 587 nm

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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