rbs+crtZW under pBad promoter for double plasmids
This part contains crtZW, which works as the astaxanthin biosynthesis pathway when add beta-carotene as a substrate.
It is necessary to add either beta-carotene or the synthesis operon.
Those work are done by Yumiko Kinoshita.
These parts contain crtW (β-carotene ketolase) under inducible promoter control. This enzyme is responsible for the conversion of zeaxanthin into astaxanthin. Our team designed two BioBricks related to this conversion. Characterization of BBa_K395706 has been performed in E. coli strain MG1655. (→more information)
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1205
- 21Illegal BglII site found at 2405
Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 979
- 1000Illegal BsaI.rc site found at 2488
Illegal SapI site found at 961
Illegal SapI site found at 2315
Illegal SapI site found at 2327