crtW (beta-carotene ketolase) with rbs
CrtW (beta-carotene ketolase) from Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271.
This is the enzyme of the carotenoid biosynthesis pathway. CrtW converts zeaxanthin to astaxanthin, the final metabolite of the pathway.
In order to confirm that BBa_K395705 is worked, we construct a BioBrick part (BBa_K395706), which contains this part under BBa_K395702. We synthesized astaxanthin by BBa_K395706, when add beta-carotene as a substrate. Thus we confirmed that BBa_395705 is working. Characterization of BBa_K395706 has been performed in E. coli strain MG1655. (→more information)
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 632
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 715
Illegal SapI site found at 542
Illegal SapI site found at 554