Apple fragrance generator
BBa_K395602 produce MpAAT1 enzyme that catalyzes production of 2-methylbutyl acetate and butyl acetate from 2-methylbutanol and butanol. 2-methylbutyl acetate and butyl acetate has a apple fragrance.
We designed apple fragrance expression device with MpAAT1. Fig. 1 shows the outline of the device. MpAAT1 converts substrate and Acetyl CoA into apple fragrance molecules. Acetyl CoA exists originally in E.coli cell. MpAAT1 converts 2-methyl butanol and butanol into 2-methylbutyl acetate and butyl acetate respectively.
We performed gas chromatography to confirm the production of the esters, and the results revealed that MpAAT1 successfully converted alcohol and Acetyl CoA into our target esters (Fig. 2).
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1765
Illegal NotI site found at 1666
- 21Illegal BglII site found at 195
Illegal BamHI site found at 1635
Illegal XhoI site found at 1675
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 205
Illegal AgeI site found at 1419
- 1000COMPATIBLE WITH RFC