Signalling

Part:BBa_K395162

Designed by: Kaneta Yusuke   Group: iGEM10_Tokyo_Tech   (2010-10-05)

CmR activated by LuxR and 3OC6HSL(AHL of Lux family)

Fig.1 Growth curve of the cell introduced PlacIq-CmR (BBa_K395165,blue curves), Plux-CmR (BBa_K395162,Red curves), or promoterless-CmR (BBa_K395160,green curves) with or without AHL (3OC6HSL).
This work is done by Yusuke Kaneta.

This device has chloramphenicol resistance (CmR) gene (BBa_K395162) downstream of lux promoter. We found that the device is activated by LuxR/3OC6HSL complex and that the cell introduced the part was able to survive even in high chloramphenicol concentration (750 ug/ml) in the presence of 3OC6HSL.(100nM)
For more information, see our work in Tokyo_Tech 2010 wiki















Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None