Reporter

Part:BBa_K395105

Designed by: Shohei Kitano   Group: iGEM10_Tokyo_Tech   (2010-10-05)

GFP reporter repressed by LuxR and 3OC6HSL (K395008:K121013)


Tokyotech R0061 K395008 graph K395008.jpg

In order to measure activity of R0061, we constructed this BioBrick combining LuxR repression promoter (K395008) and gfp reporter (K121013)on pSB6A1.


When we measured the fluorescence, we introduced this BioBrick and S03119 (pTetR-rbs-luxR-ter) on pSB3K3. The left figure shows that this promoter is repressed by 3OC6HSL and the strength of this promoter is weaker than R0061.

(→more information)









Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 64
    Illegal BamHI site found at 53
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 756


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