GFP reporter repressed by LuxR and 3OC6HSL (K395008:K121013)
In order to measure activity of R0061, we constructed this BioBrick combining LuxR repression promoter (K395008) and gfp reporter (K121013)on pSB6A1.
When we measured the fluorescence, we introduced this BioBrick and S03119 (pTetR-rbs-luxR-ter) on pSB3K3. The left figure shows that this promoter is repressed by 3OC6HSL and the strength of this promoter is weaker than R0061.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 64
Illegal BamHI site found at 53
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 756