Reporter

Part:BBa_K395101

Designed by: Shohei Kitano   Group: iGEM10_Tokyo_Tech   (2010-10-05)

GFP reporter repressed by LuxR and 3OC6HSL (R0061:K121013)

This part is GFP reporter which is repressed by LuxR and 3OC6HSL, and the promoter is R0061. And we use this part to measure the activity of BBa_R0061.

Tokyotech R0061 K395008 graph R0061.jpg

In order to measure the activity of R0061, we introduced S03119 (pTetR-rbs-luxR-ter) on pSB3K3 and K395101 into DH5α. We confirmed that R0061 is repressed by 3OC6HSL and LuxR as we expected. (→more information)










Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 64
    Illegal BamHI site found at 53
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 756


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Categories
Parameters
None