Reporter

Part:BBa_K389403

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-10-11)

Sensitivity tuner + luciferase

This is a modified BBa_I746390 sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10[luc2] vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal. The signal is supposed to be amplified 30 - 35 fold compared to a system without sensitivity tuner (Cambridge, iGEM 2007, amplifier project).


Usage and Biology

For an aplication of this part look for BioBrick BBa_K389423.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1322
    Illegal NgoMIV site found at 2666
    Illegal NgoMIV site found at 2687
    Illegal AgeI site found at 845
    Illegal AgeI site found at 957
    Illegal AgeI site found at 1159
    Illegal AgeI site found at 2390
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2572


[edit]
Categories
//cds/enzyme
//chassis/prokaryote/ecoli
//classic/reporter
//function/reporter/light
Parameters
None