Reporter

Part:BBa_K389401

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-10-11)

Sensitivity tuner + luciferase

This is a modified BBa_I746370 sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10[luc2] vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal. The signal is supposed to be amplified 15 - 20 fold compared to a system without sensitivity tuner (Cambridge, iGEM 2007, amplifier project).


Usage and Biology

For an aplication of this part look for BioBrick BBa_K389421.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1295
    Illegal NgoMIV site found at 2639
    Illegal NgoMIV site found at 2660
    Illegal AgeI site found at 818
    Illegal AgeI site found at 930
    Illegal AgeI site found at 1132
    Illegal AgeI site found at 2363
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2545


[edit]
Categories
//cds/enzyme
//chassis/prokaryote/ecoli
//classic/reporter
//function/reporter/light
Parameters
None