Luciferase from pGL4.10[luc2]
Structure of Photinus pyralis firefly luciferase. Image taken from: http://en.wikipedia.org/wiki/Luciferase.
Usage and Biology
The activity of a luciferase is measured in a luminometer - so only use this BioBrick if you have one in your lab.
Firefly luciferase oxidizes its substrate, firefly luciferin, in presence of ATP and oxygen, emitting light (Conti et al., 1996). Because the firefly luciferin does not pass the cell membrane of E. coli under "normal" cultivation conditions the cells have to be lysated before the luciferase activity measurement.
|Behaviour during cultivation||
|Kinetic of luciferin conversion||max. output between 20 - 40 s|
|Limit of detection (LOD)||162 RLU ~ 0.3 % of BBa_J23103 output|
|Limit of quantification (LOQ)||306 RLU ~ 0.7 % of BBa_J23103 output|
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 64
Illegal NgoMIV site found at 1408
Illegal NgoMIV site found at 1429
Illegal AgeI site found at 1132
- 1000COMPATIBLE WITH RFC