The VirA receptor is used by Agrobacterium tumefaciens to detect acetosyringone and other phenolic substances which are secreted by plants after injury. In presence of these substances VirA phosphorylates itself and afterwards VirG, a response regulator which activates vir promoters. These promoters control genes which are used for infecting the injured plant.
This virA gene was isolated from the TI-plasmid of A. tumefaciens C58. An illegal PstI restriction site was removed by site-directed mutagenesis.
Usage and Biology
You can find an already assembled VirA/G-receptor system here: BBa_K389017.
This receptor recognizes:
This receptor does not recognize
- Homovanillic acid
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 586
- 21Illegal BglII site found at 1571
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal SapI.rc site found at 1707