fcsR, PA2133, cyclic di-GMP phosphodiesterase
Degradation of c-di-GMP in Gluconacetobacter hansenii ATCC 53582.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal XhoI site found at 703
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 61
Illegal NgoMIV site found at 806
- 1000Illegal BsaI site found at 700
Illegal BsaI.rc site found at 448
Illegal SapI.rc site found at 813
fcsR is originated from the genome of Pseudomonas aeruginosa (PAO1), the FcsR expressed by fcsR is the phosphodiesterase (PDE) of c-di-GMP.
The encoding sequences for FcsR were inserted into the expression vector with BBa_K880005 (BBa_J23100 & BBa_B0034) to obtain J23100-B0034-fcsR-rrnB T1 (BBa_K3740062). We introduced the constructed plasmid into E. coli DH5α to verify its successful construction, and then transferred it into G. hansenii ATCC 53582 to verify its function.
As shown in Figure 2, c-di-GMP phosphodiesterase-encoded genes BBa_K3740062 was identified successfully by PCR amplification.
As shown in Figure 3, BC production in J23100-B0034-fcsR-rrnB T1-pSEVA331-G. hansenii ATCC 53582 was lower than the control group pSEVA331-G. hansenii ATCC 53582, indicating that FcsR was capable of hydrolyzing c-di-GMP in G. hansenii ATCC 53582.