Modulation of protein expression level
Sequence and Features
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Mutagenesis at specific position of RBS (BBa_B0034) was achieved RBS004 (BBa_K3740018) using random primers and PCR, and the constructed plasmid J23100-RBS004-sfGFP-rrnB T1 (BBa_K3740059). Compare the fluorescence intensity of sfGFP induced by J23100-B0034-sfGFP-rrnB T1 (BBa_K3740058).
Constructed J23100-RBS004-sfGFP-rrnB T1 (BBa_K3740059) was transformed into E. coli DH5α. We quantified the fluorescence intensity when the optical absorbance of bacterial culture at 600nm was around 0.2.
The average fluorescence intensity of sfGFP induced by RBS004 (BBa_K3740018), was less than 3% of B0034 (BBa_B0034), which means reducing the pressure to the host bacteria.
 Zhang H M , Chen S , Shi H , et al. Measurements of Gene Expression at Steady State Improve the Predictability of Part Assembly[J]. Acs Synthetic Biology, 2016, 5(3):269.