Measurement

Part:BBa_K358000

Designed by: Ken Kajita   Group: iGEM10_Kyoto   (2010-09-12)

Lac promoter with GFP

The lac promoter will be repressed and not induce GFP in the presence of lac I. This part can be used to measure the activity of lactose promoter by GFP fluorescence.

It is recommended that low copy number plasmid and E.coli strain over expressing lacI are used to measure the change of activity of lactose promoter depending on its inducer, such as IPTG.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 726


[edit]
Categories
Parameters
n/aLac promoter with GFP