DNA
HR1-GAL1

Part:BBa_K300986

Designed by: Susanna Zucca, Federica Sampietro, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-03)

Homologous region in S288C yeast genome (GAL1 ORF)

Homologous recombination can be exploited to integrate the parts of interest into S. cerevisiae genome.

Yeast integrative vectors, such as BBa_K300001, require two sequences (here named HR1 and HR2, where HR stands for Homologous Region) that are homologous with two target regions of the S. cerevisiae genome. The part of interest, contained in the yeast integrative vector, will be inserted in the genomic region comprised between the two target sequences by homologous recombination, thus disrupting the original genomic DNA between them.

This part is a 45 bp-long sequence which can be used together with BBa_K300987 in a yeast integrative vector to target the genomic region of S288C S. cerevisiae (BBa_K300979) which includes the divergent GAL1/GAL10 promoter and a small region of the GAL1 and GAL10 ORFs.

In particular, this part is homologous with a fragment of the GAL1 ORF (see Part design section for details).

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None