Cell

Part:BBa_K300985

Designed by: Manuel Lupotto, Lorenzo Pasotti, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-09)

E. coli strain BW23474 (pir-116)

Thanks to its pir-116 genotype, this E. coli strain can be used to propagate plasmids with R6K conditional replication origin (BBa_J61001, BBa_K300998), such as BBa_K300000 integrative base vector after the excision of its insert (BBa_I52002).

BW23474 propagates R6K origin at a copy number comparable to high copy plasmids (~250 plasmids per cell) [1].

Genotype:

F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am)?, endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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