E. coli strain BW23474 (pir-116)
Thanks to its pir-116 genotype, this E. coli strain can be used to propagate plasmids with R6K conditional replication origin (BBa_J61001, BBa_K300998), such as BBa_K300000 integrative base vector after the excision of its insert (BBa_I52002).
BW23474 propagates R6K origin at a copy number comparable to high copy plasmids (~250 plasmids per cell) .
F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am)?, endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC