Cell

Part:BBa_K300984

Designed by: Manuel Lupotto, Lorenzo Pasotti, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-09)

E. coli strain BW25141 (pir+)

Thanks to its pir+ genotype, this E. coli strain can be used to propagate plasmids with R6K conditional replication origin (BBa_J61001, BBa_K300998), such as BBa_K300000 integrative base vector after the excision of its insert (BBa_I52002).

BW25141 propagates R6K origin at a copy number comparable to medium copy plasmids (~15 plasmids per cell) [1].

Genotype:

F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δ(phoB-phoR)580, λ-, galU95, ΔuidA3::pir+, recA1, endA9(del-ins)::FRT, rph-1, Δ(rhaD-rhaB)568, hsdR514

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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