Measurement

Part:BBa_K300090

Designed by: Giacomo Zambianchi, Alessandro Ranieri, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-17)

Measurement system for BBa_K300094

This part was built to quantify the affinity and binding activity of a head (BBa_K300002) and internal domain (BBa_K300003) phasin separed by a flexible protein domain linker (BBa_K105012) to polyhydroxyalkanoates (PHAs) granules. With this BioBrick the right folding of the recombinant protein was also tested, by measuring the GFP reporter (BBa_K125500).

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1921


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Categories
Parameters
None