Protein_Domain

Part:BBa_K300084

Designed by: Giacomo Zambianchi, Sara Bisi, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-17)

Phasins with intein downstream (separated by a flexible protein domain linker)

Phasins (head and internal domain) work as reported in BBa_K300002 and BBa_K300003, while intein is described in BBa_K300004. This is a part composed of two phasins (head and internal domain) and an intein separated by a 10aa flexible protein domain linker, which has the function to improve, enhance and ease the folding of fusion proteins.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1696


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Categories
Parameters
None