Cm resistance and R6K replication origin
This part contains a Chloramphenicol resistance cassette and the R6K conditional replication origin.
When this part is contained in a pSB*** plasmid, it can be propagated in all E. coli strains. However, when this part is cut with XbaI-SpeI and self-ligated, it can be propagated only in pir+ and pir-116 E. coli strains (such as BBa_K300984 and BBa_K300985 respectively).
This part has been used to construct BBa_K300000 integrative vector.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC