Composite

Part:BBa_K300008

Designed by: Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi   Group: iGEM10_UNIPV-Pavia   (2010-10-05)

Cm resistance and R6K replication origin

This part contains a Chloramphenicol resistance cassette and the R6K conditional replication origin.

When this part is contained in a pSB*** plasmid, it can be propagated in all E. coli strains. However, when this part is cut with XbaI-SpeI and self-ligated, it can be propagated only in pir+ and pir-116 E. coli strains (such as BBa_K300984 and BBa_K300985 respectively).

This part has been used to construct BBa_K300000 integrative vector.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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