Engineered pH-inducible intein (codon optimized for E. coli) - internal domain
Naturally occurring inteins are protein segments that are able to excise themselves from a precursor protein and rejoin the remaining portions (the exteins) with a peptide bond.
This part is a highly engineered intein that is able to self-cleave its C-terminus at low pH. For this reason, protein domains can be easily cleaved by using this intein without the need of proteases and their cleaving sites.
The optimal pH for cleavage has been reported to be 6.0, while a pH of 8.5 has been shown to inhibit the cleavage [see reference 1].
C-terminus cleavage is also strongly affected by temperature >20°C [see reference 1 for details].
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC