Protein_Domain

Part:BBa_K300003

Designed by: Giacomo Zambianchi, Alessandro Ranieri, Manuel Lupotto, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-03)

Phasin (PhaP) - internal domain

Polyhydroxyalkanoates (PHAs) are polyoxoesters that are produced by diverse bacteria and that accumulate as intracellular granules. Phasins are proteins that can bind these granules.

This part is derived from phaP gene from Ralstonia eutropha, which encodes for a phasin, but engineered without the stop codon and with Prefix and Suffix compatible with RFC23 (Silver Standard) in order to fully support protein fusions as an internal domain.

Phasins can be used as affinity tags for a target protein, which can bind PHA granules via the phasin tag. It has been shown that mutiple phasins in tandem can increase the affinity with PHA. This part, together with BBa_K300002, enables the construction of synthetic affinity tags based on phasins in tandem, possibly spaced by peptide linkers, as described in [reference 1].


It is an update of BBa_K208001 part.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None