Protein_Domain

Part:BBa_K300002

Designed by: Giacomo Zambianchi, Alessandro Ranieri, Manuel Lupotto, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-03)

Phasin (PhaP) - head domain

Polyhydroxyalkanoates (PHAs) are polyoxoesters that are produced by diverse bacteria and that accumulate as intracellular granules. Phasins are proteins that can bind these granules.

This part is derived from phaP gene from Ralstonia eutropha, which encodes for a phasin, but engineered without the stop codon in order to support protein fusions as a head domain.

It can be used as a N-terminal affinity tag for a target protein that has to be fused downstream. The tagged protein adhere to the surface of the PHA granules via the phasin tag, which enables the target protein purification.

In literature [see reference 1] it has been shown that affinity tags composed by phasins assembled in tandem can increase the affinity with PHA. This part, together with BBa_K300003 enables the construction of such composite tags.

Because this part is a head domain, the Prefix is compatible with RFC10 and the Suffix is compatible with RFC23.


It is an update of BBa_K208001 part.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None