Composite

Part:BBa_K274382

Designed by: Vivian Mullin   Group: iGEM09_Cambridge   (2009-10-19)

PoPS -> PSP3 pag - PP promoter -> PoPS

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Device Characterisation

This device, connected to pBAD input and GFP output, constitutes part BBa_I746382. This was tested for varying concentrations of arabinose, with 9 repeats. The maximum normalised GFP rate was compared to that produced by the standard promoter device, J69591.

Characteristic Value
Increase in Transcription Rate 0.50 RPU
Basal Transcription Rate 0.03 RPU
Half Maximal Induction 4.00 μM Arabinose
Hill Coefficient 2.4

Cambridge characterisation82.png

[edit]
Categories
Parameters
None