Composite

Part:BBa_K274111

Designed by: Siming Ma   Group: iGEM09_Cambridge   (2009-10-19)


CrtY under Pbad promoter (I0500)

This construct is created by putting BBa_K118013 (rbs+CrtY) under the control of Pbad promoter (I0500), by standard assembly.

CrtY (lycopene B-cyclase) converts lycopene (red) to beta-carotene (orange). When used in double-transformation together with a constitutive lycopene-producing device (e.g. BBa_K274110), the resulting system can produce colour change (red to orange) upon induction with arabinose.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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