Other
LongLinker

Part:BBa_K243006

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-14)

Long Linker (Gly-Gly-Ser-Gly)x3

This part is a linker, it can be used to connect two parts and add additional space between them. That can be necessary to avoid interactions between these parts. We used this part to connect our protein domains with our lipocalin domains, for our project the universal endonuclease.The sequence produced the aminoacids Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly.


Usage and Biology

Peptide linkers are used to connect protein domains. For this connection it is important that the linker itself has no influence on the connected parts. Therefore the sequence of the linker is designed for amino acids which do not interact with other amino acid residues. The amino acids glycine and serine are zwitterionic and hydrophile, these properties make them a good choice for the repetitive sequence of the linker.
The length of the linker is important to guarantee the independent function of two connected parts. When the linker is too short there might be a sterical interference between the parts and when it is too long, it can cause an instability of the construct. Also, it is important that the linker has a certain flexibility.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/linker
Parameters
None