Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 606
- 1000COMPATIBLE WITH RFC
Usage and Biology
This is a fragment of BBa_K2382010. We designed a restriction site, BamHI, before EAAAK rigid linker, so future iGEM teams could take advantage of this part to fuse their proteins with RFP as indicator.
Characterization of the EAAAK+ RFP+Histag
This is an improvement of previous BioBrick Part BBa_E1010. It encodes a RFP that has
the same amino acids as BBa_E1010 and having a His-Tag at the end.
This part has codon optimized for E.coli. And we removed the barcode, which contains stop codon, from BBa_E1010. We hope this design could help other teams to utilize RFP in their fusion protein design and be easily purified.
BioBrick Part BBa_E1010