Coding
rpoA

Part:BBa_K238010

Designed by: Annelien Verfaillie   Group: iGEM09_KULeuven   (2009-07-28)

RpoA

This RpoA gene from Agrobacterium tumefaciens encodes the alfa subunit of the RNA Polymerase. This is necessary in E. coli so that the Vir box can be transcribed properly, allowing a functional VirA/VirG signaling system.

RpoA has illegal EcoRI restriction sites in its original sequence. We used site directed mutagenesis to get these sites out. EcoRI was mutated from GAATTC to GAATTT

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 978
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 951
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 607


[edit]
Categories
Parameters
n/aRpoA