This is VirA, a histidine kinase sensor originally found in Agrobacterium tumefaciens, Ti plasmid. It is part of the two-component VirA/VirG signal transduction system. This system is able to sense phenolic compounds and regulate gene transcription correspondingly.
VirA has illegal PstI restriction sites in its sequence. We used site directed mutagenesis to get these sites out. PstI was mutated from CTGCAG to CTGCAA.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 502
- 21Illegal BglII site found at 666
Illegal BglII site found at 1559
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 1405
- 1000Illegal BsaI site found at 1177
Illegal BsaI site found at 1969
Illegal SapI.rc site found at 880
Illegal SapI site found at 1651