Composite

Part:BBa_K2285017

Designed by: Zhuoyang Chen   Group: iGEM17_SJTU-BioX-Shanghai   (2017-10-19)


Constitutive promoter J23119 followed by a stem-loop structure Target1, with chromoprotein amilGFP as the reporter gene. The original part of amilGFP chromoprotein is presented on BBa_K592010. What should be noticed is, the original part is a coding part and doesn't have its own RBS and Term. Therefore, to achieve greater convenience fo our Star-chromoprotein reporter system, we add the RBS which is on the upstream of sfGFP(BBa_K515005) as well as a short terminator B1006 to all chromoproteins we used in this project via PCR.

Usage and Biology

The coding region of amilGFP was originated from iGEM11_Uppsala-Sweden.

Althought Target1 is in the STAR1 system, its structure and function are similar to those of Target3 as a terminator to stop downstream expression of any reporters. Their sequences are different from each other so we can use STAR1 and STAR3 system to control two genetic circuits.

More infomation and details about usage of this part can refer to J23119+Target3+eforRed BBa_K2285012

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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