Tar native RBS expression system (promoter+RBS+coding+terminator)
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 1355
- 1000Illegal SapI.rc site found at 184
Tar chemoreceptor is one of four native chemoreceptors of E.coli, it is mediate chemotaxis response towards aspartate and away from Ni and Co (1). This device allows the expression of a functionally native Tar chemoreceptor.
Usage and biology
This device is very similar to our BBa_K1992004 device it is only differ by its RBS. Here the expression system is using the native RBS of the Tar receptor as found in the E.coli genome (BBa_K1992000).
Expriments and results
In order to test the device it was transformed to chemoreceptor free strain (UU1250) and tested for chemotaxis ability using swarming assay. As can be seen in figure 1 the cells expressing the Tar chemoreceptor create a halo indicating a functional chemotaxis result.The larger radius of the native RBS suggests a higher expression of Tar, that might raise the sensitivity of the chemotaxis system(Figure 2) (2).
1.BI, Shuangyu; LAI, Luhua. Bacterial chemoreceptors and chemoeffectors.Cellular and Molecular Life Sciences, 2015, 72.4: 691-708.
2.SOURJIK, Victor; BERG, Howard C. Functional interactions between receptors in bacterial chemotaxis. Nature, 2004, 428.6981: 437-441.