Regulatory

Part:BBa_K199052

Designed by: Kin Lau   Group: iGEM09_MoWestern_Davidson   (2009-07-22)

pLux in reverse

This is Plux oriented in an opposite direction to typical BioBrick inserts. This Plux promoter "pointing to the left" is the same sequence (but inverted) as part BBa_R0062.

The team from Davidson College and Missouri Western State University discovered that this part promotes "backwards transcription" when LuxR protein is present and AHL-3OC6 is absent. You can read the paper that documents this unexpected "backwards promoter activity" in their open access paper.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/apLux in reverse