Regulatory

Part:BBa_K145150

Designed by: Jonas Demeulemeester   Group: iGEM08_KULeuven   (2008-08-06)

Hybrid promoter: HSL-LuxR activated, P22 C2 repressed

Hybrid promoter consisting of the Lux box upstream the -35 box and binding sites for P22 C2; OR2 and OR1 located between the -35 and -10 boxes and downstream of them respectively. Transcription from this promoter can be activated by a LuxR-HSL complex and repressed by p22 C2. Background transcription from this pomoter (no activation and no repression) is very low.

Comment

Sequence read does not match part. Part was made with two primers and Klenow end-filling. These primers are available on request.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//function/cellsignalling/LuxR
//promoter
//regulation/multiple
//regulation/negative
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
negative_regulators1
positive_regulators1