Plasmid

Part:BBa_J72008

Designed by: John Anderson   Group: Anderson Lab   (2008-07-16)

phi80 integration helper plasmid pInt80-649

This plasmid is used to integrate plasmids containing R6K origins of replication (such as part J72003) and the phi80 att site (such as part J72004) into the att site of the genome. The plasmid contains a pSC101 temperature sensitive origin, an ampicillin resistance gene, and the pir gene.

This plasmid is a derivative of the CRIM helper plasmid pAH123 (ACCESSION AY048726). A pir gene was added to the plasmid, and the procedure for performing integration was modified.

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 878
    Illegal XbaI site found at 2279
    Illegal SpeI site found at 939
    Illegal SpeI site found at 4519
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 878
    Illegal NheI site found at 884
    Illegal SpeI site found at 939
    Illegal SpeI site found at 4519
    Illegal NotI site found at 4838
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 878
    Illegal BglII site found at 2722
    Illegal BglII site found at 3416
    Illegal BamHI site found at 1263
    Illegal XhoI site found at 1576
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 878
    Illegal XbaI site found at 2279
    Illegal SpeI site found at 939
    Illegal SpeI site found at 4519
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 878
    Illegal XbaI site found at 2279
    Illegal SpeI site found at 939
    Illegal SpeI site found at 4519
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5484
    Illegal EcoRI site found at 878
    Illegal XbaI site found at 2279
    Illegal SpeI site found at 939
    Illegal SpeI site found at 4519


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