Composite

Part:BBa_J72007

Designed by: John Anderson   Group: Anderson Lab   (2008-07-16)


BamHI methyltransferase encoding CRIM plasmid

The composition of J72007, referred to in house as Bca9364 is:

{FRT}{CmR}{R6k}{FRT}{Phi80-att}{Ptet}{rbs1-A}{M.BamHI-2}

J72001.J61000.J72003.J72001.J72004.J72005.J61140.J72006

When integrated into the MC1061 genome, the product strain (MC::Bca9364) methylates plasmids at BamHI sites (GGATCC).

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 18
    Illegal XbaI site found at 1369
    Illegal SpeI site found at 1557
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1804
    Illegal SpeI site found at 1557
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 18
    Illegal XbaI site found at 1369
    Illegal SpeI site found at 1557
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 18
    Illegal XbaI site found at 1369
    Illegal SpeI site found at 1557
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal XbaI site found at 18
    Illegal XbaI site found at 1369
    Illegal SpeI site found at 1557


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