Plasmid_Backbone

Part:BBa_J428362

Designed by: Marcos Valenzuela-Ortega   Group: 2021_Engineering_Committee   (2022-06-15)


pJUMP49-2B SpecR Type IIS Level 2 vector for B modules

Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number)


JUMP Collection

Joint Universal Modular Plasmids (JUMP) is a modular vector platform for Golden Gate (PhytoBrick) and Biobrick assembly based on Standard European Vector Architecture (SEVA).

For more information please see: Marcos Valenzuela-Ortega, Christopher French, Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology, Synthetic Biology, Volume 6, Issue 1, 2021, ysab003, https://doi.org/10.1093/synbio/ysab003

Plasmid Name Origin (Copy) ABr Level Module Selection 2022 Distribution Location
BBa_J428361 pJUMP49-2A pBBR322 (Medium) Spectinomycin Level 2 A BBa_J428327 Kit Plate 1 Well 6A
BBa_J428362 pJUMP49-2B pBBR322 (Medium) Spectinomycin Level 2 B BBa_J428327 Kit Plate 1 Well 6C
BBa_J428363 pJUMP49-2C pBBR322 (Medium) Spectinomycin Level 2 C BBa_J428327 Kit Plate 1 Well 6E
BBa_J428364 pJUMP49-2D pBBR322 (Medium) Spectinomycin Level 2 D BBa_J428327 Kit Plate 1 Well 6G


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2987
    Illegal suffix found at 12
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2987
    Illegal SpeI site found at 13
    Illegal PstI site found at 27
    Illegal NotI site found at 20
    Illegal NotI site found at 2993
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2987
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2987
    Illegal suffix found at 13
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2987
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3002
    Illegal SpeI site found at 13
    Illegal PstI site found at 27
    Illegal NgoMIV site found at 1035
    Illegal NgoMIV site found at 1437
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3009
    Illegal BsaI.rc site found at 6
    Illegal SapI.rc site found at 2235


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/jump/level2
//plasmidbackbone/assembly/typeiis
Parameters
resistanceSpectinomycin