Measurement

Part:BBa_J107010

Designed by: Lorenzo Pasotti   Group: Magni_UNIPV_Lab   (2010-06-01)

R0011 measurement system (with RFP)

This part can be used to characterize BBa_R0011 hybrid promoter using RFP as reporter gene.

The hybrid lac promoter (BBa_R0011), has been designed taking the Plambda promoter (BBa_R0051) and substituting its cI (BBa_C0051) binding sites with two lacI binding sites.

This promoter can be repressed by lacI (BBa_C0012), which can be repressed by lactose or IPTG. Differently from wild type lac promoter, this part does not have any CAP binding sites, so its behaviour is glucose-independent.

Strains bearing a genomic copy of lacI can repress this promoter, which acts as a glucose-independent lactose/IPTG sensor. In the other strains BBa_R0011 acts as a constitutive promoter.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 636
    Illegal AgeI site found at 748
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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