R0011 measurement system (with RFP)
This part can be used to characterize BBa_R0011 hybrid promoter using RFP as reporter gene.
This promoter can be repressed by lacI (BBa_C0012), which can be repressed by lactose or IPTG. Differently from wild type lac promoter, this part does not have any CAP binding sites, so its behaviour is glucose-independent.
Strains bearing a genomic copy of lacI can repress this promoter, which acts as a glucose-independent lactose/IPTG sensor. In the other strains BBa_R0011 acts as a constitutive promoter.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 636
Illegal AgeI site found at 748
- 1000COMPATIBLE WITH RFC