OriT-F (Origin of Transfer for the F-plasmid nic region)
OriTf (Origin of Transfer for the F-type conjugative plasmid) is the nic region, where the relaxosome nicks the plasmid and conjugative transfer by F plasmid machinery begins via rolling circle replication.
This particular plasmid has been derived from a F-type plasmid donated by Professor Laura Frost, University of Alberta.
NOTE: OriTf is NOT the same as the host cell's native Origin of Transfer (this is a separate site within the bacterial host's main genome).
Usage and Biology
Origin-of-transfer nic region excised from Laura Frost's F-plasmid. Plasmids with this BioBrick attached to can be passed into a recipient cell (which cannot already contain an F-type conjugative plasmid) in the presence of TraJf and other conjugal-transfer genes on the F-plasmid.
- (3/06): Both BioBrick and non-BioBrick primers are available to test for colony PCR assays.
- For more information on the usage of this part, check out the Featured Parts:Conjugation parts.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 234
Illegal NheI site found at 280
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC