Composite

Part:BBa_I746382

Designed by: Stefan Milde   Group: iGEM07_Cambridge   (2007-09-26)

PSP3 pag inducing PP promoter

This construct is used to screen the level of induction of the PSP3 pag activator on the PP promoter.

Note that the I746351 does contain a RBS.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2036
    Illegal AgeI site found at 2148
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3094


Device Characterisation

Cambridge iGEM Team 2009

This device was tested for varying concentrations of arabinose, with 9 repeats. The maximum normalised GFP rate was compared to that produced by the standard promoter device, J69591.

Characteristic Value
Increase in Transcription Rate 0.50 RPU
Basal Transcription Rate 0.03 RPU
Half Maximal Induction 4.00 μM Arabinose
Hill Coefficient 2.4

Cambridge characterisation82.png

[edit]
Categories
Parameters
None