PSP3 pag inducing PP promoter
This construct is used to screen the level of induction of the PSP3 pag activator on the PP promoter.
Note that the I746351 does contain a RBS.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1205
- 21Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 979
Illegal AgeI site found at 2036
Illegal AgeI site found at 2148
- 1000Illegal BsaI.rc site found at 3094
Cambridge iGEM Team 2009
This device was tested for varying concentrations of arabinose, with 9 repeats. The maximum normalised GFP rate was compared to that produced by the standard promoter device, J69591.
|Increase in Transcription Rate||0.50 RPU|
|Basal Transcription Rate||0.03 RPU|
|Half Maximal Induction||4.00 μM Arabinose|