Measurement

Part:BBa_I20260

Designed by: Jason Kelly   Group: Endy Lab   (2008-01-08)


Measurement Kit Test of J23101

Measkit test of J23101

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706


Characterization IONIS_PARIS 2017

Group & Author: IONIS_PARIS /La Paillasse/2017

Summary: We transformed BBa_I20260 into pSB1C3 from pSB1K3 and sent it to the registry. We thought it was important because the part in PSB1C3 was not available in the registry. This part was very useful as a control for us to characterise BBa_K098997 (pCOLD-GFP) from the iGEM Tokyo 2016 team.

Method: See the bacterial transformation protocol in our wiki IONIS_PARIS 2017_Lab work_protocols.

Results:

The bacteria transformation worked, we had colonies and we then did a PCR colony (Figure 1) to verify if the GFP was transformed into pSBIC3. We picked different colonies and the positive result was observed in the well nº3 (GFP 1). In conclusion the GFP was successfully transformed into pSB1C3.

Ionis-paris-2017-HP-GFP-charact.jpeg

Figure 1: PCR colony. A) Ladder, B) mRFP protein generator, C) GFP 1, D) GFP 2, E)GFP 3.

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