Coding
lasI

Part:BBa_C0078

Designed by: Chris Walsh (Fighting Darwins)   Group: Antiquity   (2004-01-27)


autoinducer synthetase for PAI from Pseudomonas aeruginosa

coding region for lasI protein, which produces the chemical signal AI-1


Usage and Biology

214 c->t EcoRI restriction site was present. Ttc -> ttt codon (good for E. coli)

402 g->a PstI restriction site was present. Cag -> Caa codon (slightly less optimal for E. Coli)

Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 661
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 223
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/biosynthesis/ahl
//function/cellsignalling
Parameters
directionForward
ec_numnone
keggpae:PA1432
proteinlasI
swissproP33883
tagLVA