RBS (Elowitz 1999) -- defines RBS efficiency
RBS based on Elowitz repressilator.
Usage and Biology
IIT Madras 2016's Characterization
Global non-modularity towards promoters & protein coding parts and relative strength was estimated for RBSs B0030, B0032, B0034 in our modelling
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
Note: The Elowitz RBS is the definition of efficiency 1.0.
Group: Valencia_UPV iGEM 2018
Author: Adrián Requena Gutiérrez, Carolina Ropero
Summary: We have adapted the part to be able to assemble transcriptional units with the Golden Gate method and we have done the characterization of this RBS.
BBa_K2656011 is the RBS B0034 standardized into the Golden Braid assembly method. It also includes the BioBrick equivalent scar in the 3' extreme, so the insertion of this supplementary bases ensure correct spacing for the CDS expression when assembled into a TU.
Characterization of the this part was performed with the transcriptional unit BBa_K2656101 , which was used in a comparative RBS expression experiment with composite parts BBa_K2656101 and BBa_K2656102. They all were assembled in a Golden Braid alpha1 plasmid using the same promoter, CDS and terminator.
|Table 1. Optimized parameters from experimental data (BBa_K2656009 RBS).|
|Translation rate p||p = 0.082 min-1|
|Dilution rate μ||μ = 0.01631 min-1|
We have also calculated the relative force between the different RBS, taking BBa_K2656009 strong RBS as a reference. Likewise, a ratio between p parameters of the different RBS parts and p parameter of the reference RBS has been calculated.
|Table 2. BBa_K2656008 (GB BBa_B0032 RBS) relative strength and p ratio.|
|p parameter ratio (pRBS/pref)||0.398|