double terminator (B0010-B0010)
Mfold results with the two copies of BBa_B0010 and the BioBrick scar annotated.
(The top 4 results were similar. The fifth shows the two copies of B0010 with less iteraction.)
|Using primers VR/VF2 to PCR B0010 will result in excess bands.
See a full description of the problem here.