|Part assembly||System operation||Protein expression||Assembly of protein fusions||Part measurement||Screening of part libraries||Building BioBrick vectors||DNA synthesis||Other standards||Archive|
|Or get some help on plasmid backbones.|
You may find that there does not already exist a BioBrick® plasmid backbone that fulfills your requirements. You may need a different antibiotic resistance, a different copy number, or something else.
|Reshma Shetty, Drew Endy and Tom Knight developed a method and set of parts (available via the Registry) to make the process of building a new BioBrick® plasmid backbone easier. The method and part collection are described in their open-access paper, Engineering BioBrick vectors from BioBrick parts published in the Journal of Biological Engineering.|
For convenience, we also describe the approach here. Or, you may wish to jump directly to the catalog of BioBrick parts for building BioBrick vectors.
|Reshma's method for building new BioBrick® vectors relies on building BioBrick® vectors entirely from BioBrick® parts. To make the process of building BioBrick® vectors easier, Reshma designed a BioBrick® base vector (BBa_I51020). Using the based vector, you can construct a new BioBrick® vector from (almost) any replication origin and antibiotic resistance marker, as long as the origin and antibiotic resistance marker are themselves BioBrick® parts. In a nutshell, the method allows you to assembly any replication origin and antibiotic resistance marker together into the BioBrick base vector to construct a new, functional BioBrick vector. The base vector already has many of the general plasmid features that you might need in a BioBrick® vector, so use of the base vector ensures standardization and uniformity of any resulting BioBrick® vectors. (A complete list of all design features of the BioBrick® base vector is available.)|
This plasmid construction approach enables reuse of replication origins and antibiotic resistance markers available from the Registry of Standard Biological Parts. Use of the base vector to construct all BioBrick® standard construction plasmids ensures standardization and uniformity in any resulting BioBrick® vectors.
Entering new BioBrick plasmid backbones into the Registry
BioBrick plasmid parts
Browse all BioBrick plasmid parts.