Plasmid_Backbone
pSB1AK3

Part:pSB1AK3

Designed by: Registry   Group: Antiquity   (2004-07-28)

High copy BioBrick assembly plasmid

pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

Annotated APE file for pSB1AK3
Plasmid map for pSB1AK3

Usage and Biology

Kanamycin resistant up to 5000 ug/ml.

Tetracycline sensitive at 1.5 ug/ml Chloramphicol sensitive at 3.5 ug/ml (spayne and tk 6/6/05)

  • This vector is also available in the Fusion (Freiburg) Biobrick format: Part:BBa_J18902


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3168
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3174
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3168
    Illegal XhoI site found at 1035
    Illegal XhoI site found at 2061
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3168
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3168
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3183
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2207
    Illegal EcoRI site found at 3168
    Illegal XbaI site found at 3183
    Illegal SpeI site found at 2
    Illegal PstI site found at 16


[edit]
Categories
//plasmidbackbone/assembly
//plasmidbackbone/copynumber/high
Parameters
chassis
copies100-300
insertNone
mcsBioBrick
originpMB1
resistanceAK